top student at medical school

Chapter 50 We Don't Need to Be Friends In the First Place (Please add to your favorites and read on)

"Did you make these?" Yuan Weihong asked, pointing to the label FZY 1016 on the six-well petri dish.

"Hmm, Master, it should be fine, right?" Fang Ziye was adjusting his mask at the moment.

Summoned by the teacher, Fang Ziye could only rush to the laboratory as fast as he could, not daring to delay even for a moment. At this moment, Fang Ziye didn't even have time to check whether his slippers were worn properly.

Well, in principle, you can't wear your own shoes inside the cell culture room. You need to buy a separate pair of shoes and place them on the shoe rack outside the cell culture room for later use.

Yuan Weihong examined it several more times, focusing on the view through the lens: "This is how you did it for your first time?"

"Genius?"

"Who did it for you?" Yuan Weihong asked, his mind already preoccupied with the question.

“Master, I really made this myself. How could the senior brothers in the lab have time to help me? However, Junior Sister Luo Tingzhu did give me some pointers, but she didn’t help.” Fang Ziye had already guessed that his operation was probably not bad, so he felt relieved.

But when I looked down at my feet, it seemed that these slippers weren't actually mine.

Looking towards the entrance, Fang Ziye noticed a head peering into the cell culture room through the doorway; it looked like Luo Tingzhu.

Fang Ziye had no choice but to stretch out his foot.

He arrived in such a hurry that he thought there was a problem with the cells in his scratch experiment and that the teacher had discovered it, so he grabbed a pair of familiar slippers without even looking at the small name tag.

The slippers in the lab are all pretty much the same; you wouldn't be able to tell them apart without looking at the name tags.

The cat's head immediately shrank back.

Yuan Weihong also noticed Fang Ziye's subtle movements and thought about it carefully: "Whose shoes are you wearing?"

“Your shoes are on my feet.” Yuan Weihong had spent time in the lab before, so he naturally knew the corresponding rules.

Fang Ziye swayed back and forth a few times: "Master, it's alright."

"The scratch test is usually done by taking photos at 0, 6, 12, and 24 hours. Why didn't you follow the standard?" Yuan Weihong turned and asked.

This was also the purpose of his summoning Fang Ziye. October 16th was yesterday, and it was obvious that Fang Ziye had not properly observed and taken photos.

"Master, giant cell tumors of bone grow relatively slowly, so can't we delay taking pictures?" Fang Ziye asked.

"You think it grows slowly just because you say so? Are the pictures published in the article all the original materials?"

"If you don't come, are you just waiting for someone else to clean up the mess for you? Even if the data is blank, invalid, or meaningless, isn't it experimental data?" Yuan Weihong seemed to have found an opportunity to give him a dressing down, and pressed him with this question.

Fang Ziye immediately looked slightly deflated.

Fang Ziye hadn't really paid much attention to this point. The main reason was that when he looked at other literature related to giant cell tumor of bone, the records were all observations at intervals of 24h, 48h, and 72h, rather than data records of six hours or absolute 12 hours.

Even if you add points and improve your operational skills, you will still suffer if you lack knowledge and experience.

"Master, I was wrong. I'll do it again." Fang Ziye was honest and admitted his mistake immediately.

"You're asking me to redo it for free?"

"Is the government allocating funds for research projects so that you can fail whenever you want?"

Yuan Weihong continued to criticize: "To put it mildly, this is a lack of rigor in your work; to put it more seriously, you are squandering the country's financial resources!"

Yuan Weihong carefully collected the three six-well plates and handed them to Fang Ziye, asking Fang Ziye to disinfect the surface before placing them into the cell culture incubator.

Fang Ziye then became obedient. However, after Fang Ziye finished placing the six-well culture dish, he said, "I'd like to invite Luo Tingzhu to dinner sometime. She's just a young girl, but she's been guarding our group's small cells for so long."

"Thank you properly."

Fang Ziye subconsciously tilted his head to the side, a habitual micro-movement that occurred when he suddenly started thinking.

"Do you know how to perform the Transwell cell invasion experiment?" Yuan Weihong asked as he walked towards the door.

Fang Ziye nodded.

"Really?" Yuan Weihong took off Fang Ziye's cell culture room slippers, his movements pausing slightly. Clearly, Fang Ziye's answer had left him speechless.

He came to the lab today not specifically to supervise Fang Ziye's experimental procedures, but because Luo Tingzhu, this little girl, came to him for help. The main reason was that Luo Tingzhu had asked around but no one was available to teach her, so she asked Yuan Weihong.

Yuan Weihong also had a deep memory of Luo Tingzhu, so he thought he would come over to take a look when he had nothing to do. He also checked on Fang Ziye's experimental progress and found that Fang Ziye's operation was exceptionally good, but there were also flaws.

"It should be about right." Fang Ziye nodded, his tone calm and composed, not daring to be presumptuous.

"What are the experimental procedures?" Yuan Weihong didn't want Fang Ziye to be domineering.

Criticism is criticism, and carelessness is carelessness.

Damn, he's only been in the lab for less than half a month and he's already mastered cell scratch assays. Such talent is rare, if not nonexistent, but certainly not exceptional.

After all, it takes most people a while to learn how to use laboratory equipment, copy experimental records, and culture cells.

In Yuan Weihong's eyes, Fang Ziye has always been a diligent and reliable student, rather than a naturally gifted player.

Fang Ziye's previous gradual progress was the result of years of hard work and gradual accumulation, not a sudden leap forward. Now, however, he has made rapid progress in such a short time since entering the lab, which has truly left Yuan Weihong bewildered.

We're master and disciple, who doesn't know who the other is?

Teletubbies.

"The general process is: cell culture and processing—coating with a basement membrane—hydrating the basement membrane—preparing a cell suspension—inoculating cells into the upper chamber—cell fixation—cell staining—mounting—cell observation and counting," Fang Ziye replied slowly.

Although the answers were slow, they were precise.

"What's the experimental principle?" Yuan Weihong returned the shoes to Fang Ziye.

"The invasion experiment involves placing a small chamber into a culture plate. The small chamber is called the upper chamber, and the culture plate is called the lower chamber. The upper and lower culture media are separated by a polycarbonate membrane. The upper culture media is added to the upper chamber, and the lower culture media is added to the lower chamber. Cells are seeded in the upper chamber. Because the membrane is permeable, the components in the lower culture media can affect the cells in the upper chamber, thus allowing us to study the effects of the components in the lower culture media on cell growth, motility, and other processes."

"But when tumor cells are starved, they will definitely try their best to penetrate into the lower culture medium. The number of tumor cells that reach the lower culture medium represents their invasive ability."

"Just like in high school, whoever is hungrier runs faster. Everyone is equally hungry, so whoever runs faster gets to eat first," Fang Ziye added, drawing a parallel.

Yuan Weihong's lips twitched, though his face was covered by a mask, his eyes showed a troubled expression: "You'll have no friends if you keep going like this."

“Master, we are master and disciple, we don’t need to be friends.” Fang Ziye grinned, knowing that he had passed this hurdle and would not be criticized by Yuan Weihong any further.

"You don't take the phrase 'both teacher and friend' seriously, do you?" Yuan Weihong's tone was full of jealousy.

Those who haven't spent time in a laboratory simply cannot understand the hatred Yuan Weihong felt for someone like Fang Ziye at this moment.

Screw you, why don't you suffer the beatings that cell experiments bring you? You're so accurate every time you do it, tumor cells are your relatives!

(End of this chapter)