top student at medical school

Chapter 37: Unprecedentedly Good (Please add to your favorites and read on!)

"Senior Brother Fang, could I borrow your PBS? Mine might be contaminated. My cells were growing perfectly fine last time, but after rinsing with PBS, they all died this time." While Fang Ziye was deep in thought, Luo Tingzhu probably checked her culture dish again at some point.

PBS buffer is like a bath for cells. When cells adhere to the culture medium and grow, they are rinsed once after the original culture medium is replaced.

If the PBS buffer is contaminated, that would be a disaster.

Compared to bacteria, tumor cells are nothing!

And if the cultured cell line gets infected, it's even more heartbreaking than if you yourself get injured.

The tiny cells cultured in the laboratory are the same as the corn and sweet potatoes grown in the ground by agronomy PhDs. They are extremely precious.

Fang Ziye was startled upon hearing this and turned to look at Luo Tingzhu, whose brows were slightly furrowed: "Junior Sister Luo, did you lose the petri dish? Can I take a look?"

Fang Ziye was like a curious child.

Fang Ziye had never seen what a polluted field looked like, and he wanted to guess what it looked like to increase his knowledge points.

If possible, this laboratory would be a vast treasure trove.

Luo Tingzhu nodded blankly, understanding Fang Ziye's feelings: "Then PBS?"

After the contamination, a possible cause was found, and a new order was needed. Luo Tingzhu may have prepared the subsequent experimental procedures. Now that the cells are gone, it's like the experiment has no subjects. It's like wanting to go out to eat, but there's no restaurant to be found.

"It's alright, Junior Sister Luo, you can take it and use it. Just seal it up when you're done." Fang Ziye quickly put on a sterile mask and cap and ran into the cell culture room.

Reagents used in the laboratory must be sealed with a film after use to prevent them from being easily contaminated by bacteria.

Luo Tingzhu's PBS buffer may have been improperly sealed.

When Fang Ziye arrived at the cell culture room, he saw three culture dishes labeled LTZ 10.08, which meant that Luo Tingzhu changed the medium on October 8th, or that the cells were transferred again.

It's for cell experiments; don't use it indiscriminately.

Fang Ziye's label is FZY 10.10, which is convenient for everyone to distinguish.

Fang Ziye picked one up and placed it under the microscope. Sure enough, he saw that the adherent cells had lysed, and various impurities and debris were present in the suspension.

Since the petri dishes are separated, an oil immersion microscope cannot be used, and bacteria cannot be seen. Nor can other common contaminants such as Bacillus cereus contamination be observed. Therefore, it can be inferred that the contamination is bacterial.

Upon closer inspection, Fang Ziye indeed discovered that his knowledge points had rapidly increased by 3 points.

Perhaps. The cell line that Luo Tingzhu is currently cultivating has also undergone special treatment. It is a special cell line that is rare worldwide and does not have a mature theoretical system.

Even if such cell lines are contaminated and die, they can still help Fang Ziye understand what the dead and lysed states are like, which is a new kind of knowledge.

There is knowledge to be gained everywhere.

Fang Ziye quickly changed to another one, and then another one.

I found that everything I saw was pretty much the same, and I didn't gain any more knowledge.

"Too greedy, too greedy," Fang Ziye could only mutter to himself.

As Fang Ziye watched, he saw that Luo Tingzhu had prepared all the cell resuscitation reagents and equipment that Fang Ziye had prepared earlier. She had also carefully put the PBS and culture medium into a 37°C constant temperature water bath to preheat them.

The goal is to simulate the normal temperature inside the human body as closely as possible, so that the tumor cells can grow healthily and happily after revival, and then become useful experimental materials for Luo Tingzhu.

"Junior Sister Luo, should I help you throw these things away?" Fang Ziye asked.

"Just throw it away. I asked my senior, and it's so contaminated that it's beyond saving. I was even planning to do a scratch test."

"Sigh!" Luo Tingzhu said something that was a bit beyond the scope of Fang Ziye's current experimental progress.

Fang Ziye didn't immediately delve into the matter. Instead, he took another curious look at his cell culture dish and found that the cells were still suspended and hadn't adhered to the wall. He realized that he had been too hasty.

After returning the cells to the incubator, Fang Ziye bid farewell to Luo Tingzhu.

Luo Tingzhu doesn't need his guidance on cell resuscitation, so staying here would be a joke and only add to her stress... Fang Ziye has only entered the laboratory, not in a full-time or part-time capacity. Therefore, Fang Ziye still needs to take care of the patients in the clinic. On Monday, the day of surgery, Fang Ziye still needs to go and see if there is an opportunity to work on them.

Monday was a relatively lucky day.

Although Professor Deng Yong and Professor Xie Jinyuan continued to push forward the surgical schedule with all their might, Fang Ziye was not deprived of the opportunity to perform surgery on the patients under his care.

Furthermore, during the afternoon surgeries, one of them was for my junior brother Jie Han, and after the surgeries were split up, my senior brother Sun Shaoqing was going to perform surgery on another platform. Therefore, Fang Ziye had the opportunity to assist on two platforms.

On the first machine, Fang Ziye only served as an assistant; he didn't get to touch any of the other operations, as Professor Deng Yong and his own teacher had already mastered them all.

Finally, after the tables were divided, Fang Ziye, with the help of his mentor, completed a cutting technique to maintain his feel for the job.

At this moment, Yuan Weihong's feelings were still focused on Fang Ziye's glorious family reputation. After reading it, he said, "Even if you're in the lab, don't neglect clinical operations. Occasionally, you should also go to the practice room and the operating room."

Jie Han said, "Master, I heard from the other senior brothers that Senior Brother Fang spent two hours in the practice room last night and didn't go back until after 11 p.m.

"Where did you get so much hearsay from?" Yuan Weihong asked, turning his head to the side.

Jie Han's eyes flickered rapidly a few times before he replied, "Master, thanks to your excellent teaching and my senior brother's support, I've published several more articles, so everyone among my fellow disciples is quite respectful."

"Some people have asked me to teach them how to write a meta-analysis, but I haven't dared to agree."

Nearly three-quarters of Fang Ziye's research output was done in collaboration with Jie Han. Jie Han published one article independently, one article co-authored with Fang Ziye, both of which have been published, another letter, and one revised article, which is about to be published.

This scientific research output can only be described as incredibly impressive.

Furthermore, Jie Han has conducted a separate meta-analysis, which is nearing completion. It is also said that while he is currently practicing in his studio, he is collecting some past clinical data to conduct a retrospective clinical research analysis, and is currently in the process of data collection.

This research output is absolutely insane.

How could I not love it?

"Virtue!~"

"Any progress in the suturing technique? If so, let's suturing one machine?" Yuan Weihong looked at his two precious apprentices, raising an eyebrow in a pleasant mood.

“Master, just a little bit more, just a little bit more and I’ll be able to put the rubber skin back together, and the incision isn’t far off either.” Jie Han replied, as if to reassure Yuan Weihong that his research time hadn’t delayed his clinical progress.

Yuan Weihong felt instantly relieved and shouted, "Circulating doctor! Anesthesiologist!"

The anesthesiologist was playing on his phone when Yuan Weihong yelled at him. He shuddered, instinctively checked the vital signs, and found them all stable. He then shouted back in a thick Northeastern accent, glaring at Yuan Weihong, "What are you doing?"

"Let's have dinner together tonight!" Yuan Weihong continued loudly.

"Eat your food, eat your food, why are you shouting like that?" The anesthesiologist's voice softened.

But immediately, he put his phone away and lowered his voice: "Brother Wei, where should we go to eat?"

While performing the surgery, Yuan Weihong discussed with the circulating and anesthesiologists where to eat.

Jie Han carefully studied the details of Yuan Weihong's operations on the operating table, using one hand to help while the other hand moved nimbly, as if he were pondering the process.

Fang Ziye felt that the light from the shadowless lamps didn't seem as strong, and he felt a sense of unprecedented comfort.

Most importantly, at the end of the surgery.

The "Knowledge Points +0.4" indicator made Fang Ziye feel even more cheerful.

The book's pace is a mix of daily routines, surgeries, outpatient visits, and laboratory work. Readers should be aware that while there may be occasional exciting moments, this is the general rhythm and pace for the most part.

Please don't rush me. Slow and steady wins the race. I'll try my best to make every word I type as easy and pleasant to read as possible.

Please note again that the research pathways described in this article should not be directly correlated with reality. Thank you. Also, please do not ask the author if something has already been done or if something is impossible. The author is unlikely to publish any pathways they have actually worked on.

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(End of this chapter)