top student at medical school

Chapter 36 Pushing open the door reveals the misty, worldly realm (Please add to your favorites and read on)

Note: Some cell experimental pathways mentioned in this article differ slightly from real-world pathway guides. This is for three reasons: first, to avoid leaking research papers from our research group; second, to prevent professional colleagues from pointing out errors; and third, this novel is primarily for entertainment and is not a research paper database. Please be aware of this.

For almost every basic medical researcher, the first step on their research path is cell resuscitation technology.

Basic medical experiments involve various interventions on tumor cells or other cells from a cellular perspective to observe their activity, function, reproduction, and other characteristics.

The goal is simple: to hope that our treatment can intervene in these cells and potentially become a new direction for drug therapy for the diseases represented by these cells.

You can't just attack someone directly!

"Thaw the cells, wash them, and culture them. Thaw, wash them, and culture them." Fang Ziye first went through the routine in his mind, then turned back and smiled at Luo Tingzhu.

But Luo Tingzhu didn't laugh at Fang Ziye; instead, she encouraged him by raising her fist at him.

To conduct disease-specific research, you first need cells corresponding to that disease. Professor Yuan Weihong has already purchased these cells and cryopreserved them after differentiation.

The beginning of cell experiments involves thawing a portion of frozen cells, culturing and propagating them, and using them as experimental material for subsequent experiments.

It is usually stored in liquid nitrogen.

After removing the cell sample from the frozen state, Fang Ziye immediately took the cryovial out of the liquid nitrogen tank, which was steaming like a fairyland, and placed it in a water bath at a preset temperature of 37°C. He then gently shook it until the sample was completely thawed.

The faster this process is, the better, because some reagents in the cryopreservation solution can cause cell lysis and are not suitable for cell growth. If the process is too long, the cells are more likely to die.

If all the cell lines are used up by Fang Ziye, then Yuan Weihong will need to spend money to purchase them again.

He'll probably get a scolding.

Fang Ziye worked very quickly. After thawing, he quickly put the cryopreservation solution and cells into the culture medium, and then used the culture medium to dilute and wash the cryopreservation solution.

This is similar to how, when someone is about to be poisoned by carbon monoxide, they must immediately go to a place with fresh air to expel the toxins while preventing them from further invading the body.

Since it was the first time performing this procedure, Fang Ziye followed the best principle: he diluted the frozen culture medium into a suspension, centrifuged it once, then discarded the suspension in the test tube, leaving only the adherent cell lines, before re-mixing it with culture medium.

Take the suspension and add it to a pre-prepared petri dish.

"Senior Brother Fang, you're so extravagant! If this were in the internal medicine lab, you'd get scolded for being this extravagant." Luo Tingzhu clicked her tongue slightly.

Even well-prepared culture media are expensive because they contain serum; each milliliter can be measured in RMB.

Fang Ziye's centrifuge tube probably wasted at least ten yuan.

For a simpler and more direct approach, after removing the cells from the cryopreservation solution, the cryopreservation solution inside the cells is directly diluted with culture medium, or the cells are directly added to the culture medium.

"It's our first time, so let's just let it go and avoid killing the cell line."

"After covering the culture dish, shake it for a few days and let it grow naturally for a while. Once it adheres to the walls and covers the entire surface, you can harvest the cells, right?" Fang Ziye asked, turning around.

The cell lines that Yuan Weihong preserved are all treasures; they are basically mother lines that can be cultured and passaged for several generations before being used in experiments, without any problems.

These cell lines are quite expensive, and Fang Ziye dared not waste them.

First, culture one bottle, then freeze some, conduct experiments on some, and then perform further experiments on the next few generations of this mother plant.

“Yes, I thought you, Senior Brother Fang, were planning to do it that way. After all, Professor Yuan’s research funding is substantial, and he’s very wealthy.” Luo Tingzhu smiled.

Fang Ziye quickly shook his head. "Junior Sister Luo, have I already spent almost a hundred yuan today?" Fang Ziye asked again.

Luo Tingzhu examined the items: "The petri dishes and Professor Yuan's high-grade pipettes cost one or two yuan each. With the culture medium, it's less than a hundred yuan, but around seventy yuan is more appropriate."

"I can't afford to mess with them!" After spreading the cells evenly, Fang Ziye first examined them under a microscope.

After adjusting the focus, I found that all the cells in the field of view were round and floating in the culture medium, without any deformed or cracked cells or swimming bacteria, which made me breathe a sigh of relief.

However, at this moment, when Fang Ziye observed the movement of the cells more carefully, he suddenly discovered that his knowledge points were increasing rapidly.

"+0.1!"

"+0.3!"

"+0.4!"

About half a minute later, Fang Ziye's knowledge points increased by 5 points.

This surprised Fang Ziye slightly, leaving him somewhat puzzled.

Luo Tingzhu had already left, and Fang Ziye was also wearing a mask, so his astonishment and excitement had no one to share with him.

I placed the culture dish into the cell culture incubator, emptied the waste from the cell culture workbench, and disinfected it again with alcohol. I then took out all my experimental equipment and put it into the refrigerator and drawer in the laboratory.

Fang Ziye sat down again on a round stool in the laboratory, slightly lost in thought.

In the distance, Luo Tingzhu was already crawling on the ground, copying something. Further away, some of Professor Deng Yong's academic doctoral fellows were typing on keyboards, seemingly dissatisfied with the data they were processing.

Further away, a junior master's student was following a senior student Fang Ziye didn't recognize as they went in and out of a laboratory, asking, "Senior, what are we going to do next?"

At this moment, Fang Ziye finally understood some things.

In clinical practice, the diseases and changes we see are already based on very mature diagnostic, treatment, and surgical protocols and procedures.

In the lab, you could say that if someone has already worked on a certain area, you won't be able to get a grant. But if you do get a grant, it's always for a new area or field.

Regardless of whether this direction is useful or not, even if the verification fails, it's still a failure that no one else has verified before.

What are knowledge points?
The knowledge points you learn, right? At least that's what Fang Ziye himself thought. There may be subtle gains in treating and operating on patients in the clinic. The knowledge gained from diagnosis and treatment is considerable. It allows you to truly experience the process, and there are bound to be areas where you are not yet mature.

And the things in the laboratory, every processing method, may be something that no one else has ever done before.

For example, the cell line I revived was one that Yuan Weihong had specially treated with reagents; it seemed to be a cell line with a silenced HK2 gene. Therefore, even though I only observed its cell movement under such circumstances...

That is.

New knowledge.

Fang Ziye suddenly felt as if he had found the door to a new world, and beyond this door lay the human world, the misty rain.

What is knowledge?

New knowledge, knowledge that one is unaware of, knowledge that no one else knows, the process of creating something from nothing—all of that counts as knowledge…

(End of this chapter)