top student at medical school

Chapter 86 The Endless Treasure Trove of the New Cell Line (Second Update, Please Subscribe)
Of course, these preparations are not limited to what Fang Ziye is currently discussing with him.

Other arrangements were merely discussed rather than documented in writing.

For example, Fang Ziye has already planned for the next year. He expects to complete all the experiments for his research project by April, submit the paper, and strive to publish it during his doctoral studies to meet the basic dissertation requirements for graduation.

Meanwhile, the research results of another more in-depth project will be used for his own general research grant application and to see if Fang Ziye, who will become a postdoctoral fellow next year, can apply for municipal or provincial-level research projects under his own name.

The more basic experimental data will serve as the foundational material for my junior colleague Jie Han's application for school-level and city-level research projects. If this material is successfully developed, then starting next year, our research group will have four special research grants.

It is estimated that there will be about 500,000 yuan in research funding. At that time, the group will be relatively well-off. With so much money, Fang Ziye can conduct larger experiments, more operations, and more in-depth research in basic molecular biology and even genetic epigenetics...

Fang Ziye had truly put in the effort and planned for the future, which was why Yuan Weihong was reluctant to let him work for someone else.

How can I feel at ease not having such a good student by my side?
Naturally, the path to becoming a Thousand Talents Program or Outstanding Young Scientist is relatively smoother when you are self-employed.

“No, we can’t just let Ziye ask for money. I need to ask the hospital for some research funding.” Yuan Weihong muttered to himself, his mind racing.

The hospital has special research funding rewards. If you do well in your academic work, you can be allocated a lot of start-up funds for your projects and special research expenses.

As long as you have enough output, it's not impossible to get research funding ranging from tens of thousands to tens of millions.

Yuan Weihong planned to glance over here to see if he could squeeze some money out of Fang Ziye instead of letting him freeload here and there. If he didn't need to freeload, he could openly buy whatever he wanted, so that Fang Ziye could have more freedom to spend.

As teachers, building a higher and better platform is extremely beneficial for the development of students and the team.

For example, the laboratory platform was built by Professor Deng Yong, which is why Fang Ziye has the foundation to rely on so much. Otherwise, if you were alone and didn't even have a laboratory, what kind of experiments could you possibly do?

……

After finishing his second-to-last sentence with Yuan Weihong, Fang Ziye didn't have time to reply to his master.

At this moment, Luo Tingzhu came out of the cell culture room and said, "Senior Brother Fang, could you take a look at this cell line I received and see if there's anything wrong with it?"

"Is there a problem? Is it 143B or the HuO9 cell line?" Fang Ziye's eyes flickered.

These two cell lines were obtained by Luo Tingzhu after so much effort. If there are any problems with them, then the other party would be too unfair.
“It’s the second cell line. The shape looks a bit off to me, but it doesn’t seem to be contaminated.” Luo Tingzhu simply changed her shoes, without taking off her mask and hat.

Upon hearing this, Fang Ziye quickly put on a mask and hat, squinted his eyes rapidly, and followed Luo Tingzhu into the cell culture room.

At this moment, Luo Tingzhu placed both cell culture dishes under the microscope. The 143B type does resemble osteosarcoma in appearance, but the other cell line, the HuO9 type osteosarcoma cell line, does have a slightly different appearance from common osteosarcoma cell lines.

Although Fang Ziye himself does not work with osteosarcoma cells, he is familiar with the morphological characteristics of osteosarcoma cells.

Oval or fibrous: Osteosarcoma cells are usually oval or fibrous in shape, which allows them to attach to the surface of the culture dish and form a single or multiple layer of cells.

Mesenchymal-like characteristics: Since osteosarcoma usually originates from bone tissue, osteosarcoma cells sometimes exhibit a mesenchymal-like morphology, including elongated, stellate, or spindle-shaped.

Nuclear size and shape variation: The nuclei of osteosarcoma cells may show variations in size and shape, and this nuclear heterogeneity may be one of the characteristics of osteosarcoma.

Protrusions and pseudopodia: Osteosarcoma cells may develop protrusions and pseudopodia during culture, which helps them move and attach on the culture dish.

Heterogeneity: Different osteosarcoma cell lines may exhibit certain morphological differences. These differences may reflect osteosarcoma subtypes, as osteosarcoma can present in various subtypes, including well-differentiated and poorly differentiated types…

However, in this cell line, it doesn't seem to have the shape of osteosarcoma; it looks rather round.

Furthermore, because Fang Ziye had previously changed the IV fluid with Luo Tingzhu, and might have been partially involved, the moment Fang Ziye saw this thing, it seemed to float before his eyes as if he had gone mad:

"+1!"

"+1!"

"+1!"

"..."

This continued for more than twenty seconds, and at a rate of 2-3 points per second, a total of forty or fifty knowledge points were suddenly and directly poured into Fang Ziye's attribute panel.

Very bald.

Fang Ziye looked around, slightly bewildered, and finally his gaze settled on Luo Tingzhu's neck.

However, Luo Tingzhu did not find Fang Ziye's gaze to be aggressive at the moment, and Fang Ziye's bewildered expression confirmed her guess.

However, Luo Tingzhu asked with a hint of disappointment and nervousness, "Right, senior brother? This cell line shouldn't be an osteosarcoma cell line. I wonder what it is."

"Did that person give me the wrong thing?"

Fang Ziye then looked at the adherent growth results of another 143B cell line. It was somewhat similar to the HOS cell line that Fang Ziye had previously looked at, but there were slight differences in the details, which may be due to different subtypes.

Fang Ziye stood up: "It's possible that the wrong one was picked up. This doesn't look like an osteosarcoma cell line, and it might not even be osteosarcoma."

"Take a picture and ask her about her specific situation."

"Don't throw the petri dish away yet," Fang Ziye added later.

Goodness, I have no idea what this thing is. I only looked at it for about twenty seconds and watched its cultivation process, and I've already gained so many knowledge points. This must be something special.

Fang Ziye really wanted to preserve it, and then, without any ulterior motives, to do some intervention to see if he could gain more knowledge.

Currently, it's just being cultivated. After observing its form adhering to the wall, Fang Ziye can't even imagine what it would look like if it were processed.

Fifty knowledge points, that's one-tenth of a level 4 skill.

The awesomeness and power of level 4 skills are self-evident!

"Senior brother, I definitely won't throw it away, but this shouldn't be an osteosarcoma cell line, right? I'm not mistaken." Luo Tingzhu asked, pointing to the culture dish.

In fact, to determine what kind of cells such a cell line is, a genetic test or a comparison with a cell bank at a company can provide the answer.

But such an expense would be better spent on buying a few new cell lines. How could Luo Tingzhu possibly waste such spare money?
The research group wouldn't waste it like that.

"It shouldn't be. Take a picture first, ask that person what's going on, and then find a picture of the common cell morphology of HuO9 online. You can find it on the company's official website. Send it to her."

"Perhaps she made a mistake. If she did, we'll think of another way."

If this is indeed a mistake, then going to Shashi again would be too much trouble. Fang Ziye thought it would be better to look for it in the orthopedics departments of several hospitals affiliated with Huazhong University of Science and Technology or the Provincial People's Hospital.

Luo Tingzhu doesn't have a lot of classmates, but Fang Ziye has quite a few. Some of them went from Tianjin Medical University to other universities in Wuhan, and others are pursuing graduate studies in places like Shanghai.

Perhaps we could borrow a cell line from them and send it over by express delivery.

Since we know each other, I feel a little more at ease.

Luo Tingzhu quickly took a picture and then started asking questions.

I did not delete the friend.

Furthermore, after Luo Tingzhu sent the message, the other party replied: "Huh? Why is it this cell? Maybe I took the wrong one."

"So what do we do now? Are you going to come to Shashi to pick it up again?" the other party asked.

Luo Tingzhu showed the information interface to Fang Ziye, her expression full of desolation.

"Senior brother, it really was the other party who took the wrong one." Luo Tingzhu sighed.

Fang Ziye said, "Why don't you ask them what kind of cell it is, what kind of cell line it is? There's nothing we can do if they picked up the wrong one, that's how it is now." "If it's a cell line or cell line from our orthopedics department, we'll freeze it first, we might need it later."

The variety and quantity of cells in a laboratory is a kind of foundation.

There are many cell experiments that can be performed in orthopedics, such as those involving inflammatory cells, giant cell tumors of bone, chondroblastomas, etc.

Even cell lines that are not from orthopedics can be preserved first.

Fang Ziye can use it himself, anyway, he's already been trained.

After asking, Luo Tingzhu didn't show Fang Ziye the information again. Instead, she said, "They said that this is a new cell line that their team has constructed. It's okay if they took the wrong one. Their research group has many other cell lines, so there's no need to return it."

"It's HuO9. How do I get it to me now? I can go get it again, or they can come and deliver it."

Fang Ziye thought for a moment and said, "If she gives it to me, that would be the best."

"The team that can build new cell lines must be quite large. They might actually be students from Xiangya Hospital affiliated with Central South University who just accidentally picked up the wrong one."

“If the label falls off during cryopreservation, it is possible to mistakenly take the revived sample.”

Luo Tingzhu said, "It's actually quite troublesome to have her make a special trip to deliver it. I'll have her mail it over. Anyway, we've met in person, so I know she's not lying."

"We're doing this for research purposes..."

Luo Tingzhu then continued editing the information.

The reason why cell replacements cannot be sent directly is that cell experimental lines and cell strains can only be used for experimental and scientific research. If you are not engaged in scientific research, we cannot give them to you.

Imagine if such tumor cells were in the hands of an ordinary person and simply thrown into their food; no one could be sure what would happen in the end.

If you are exchanging with someone, at least the other party has professional equipment, and there is a high probability that the other party is engaged in scientific research. In this case, completing the mutual gifting is more reasonable in the scientific research field.

"This is a cell line for Ewing sarcoma, a cell line constructed by their team themselves. It is a typical cell line taken from the patient's body and has not yet been included in our country's national cell bank."

"We can't do experiments yet, but if we freeze it, it might be useful in the future." Luo Tingzhu also found out the source of the cell line.

"Then let's freeze it for now. It seems that no team in our lab is currently conducting experiments related to Ewing sarcoma!" Fang Ziye was delighted upon hearing this.

As expected, this must be something new. Otherwise, it wouldn't be scientific to gain so many knowledge points just by looking at cell morphology.

Wow, being able to construct a new cell line, that's quite impressive.

Fang Ziye has not yet learned how to construct a cell line, but he knows how complicated the procedure is and how complex the steps and processes are.

But that's okay. If someone else has already built it, and it gets approved later, we can use it directly, which is equivalent to saving some money.

Ewing sarcoma, right? Fang Ziye felt that he might be able to change a small experimental direction and develop it in the direction of Ewing sarcoma.

It wasn't that Fang Ziye was half-hearted; it was mainly because he gave out a lot of knowledge points!
Fang Ziye offered to help with the cell cryopreservation, but Luo Tingzhu naturally declined, saying only, "Senior brother, it's alright, I can do it myself. The procedure isn't complicated."

"This is my senior, so she's your junior, her name is Xie Xin."

She said she would send me a bottle of cultured HuO9 cell line as soon as possible.

"Senior Sister Xie Xin said that she was originally culturing the U2OS cell line, but the liquid nitrogen tank she used to freeze the U2OS cell line was empty for the entire National Day holiday."

"She went home, assuming there were other people in the lab, so..."

Fang Ziye recalled Luo Tingzhu's explanation and felt a pang of heartache for the other party's laboratory.

Holy crap, the liquid nitrogen tank was empty for a while, and the cells inside, even if they didn't die, were at least shed a layer of skin. How many cells died all at once?
Moreover, the fact that one liquid nitrogen tank is empty means that the other party may have two or three liquid nitrogen tanks, which reflects the scale of the laboratory.

As expected of Xiangya Hospital, a long-established top-tier institution with deep pockets, it's incomparable.

"Well, it's good that we've confirmed each other's identities. Having another senior sister in the same field is actually not bad. We can exchange cell lines in the future."

"Or perhaps we could buy a little bit." Fang Ziye praised Luo Tingzhu for her good luck.

With this additional connection, especially within the same field, if they get along well, Luo Tingzhu might have more resources and connections than he does in the future.

Luo Tingzhu had already returned to the cell manipulation table, turned around and smiled at Fang Ziye with narrowed eyes.

Fang Ziye wisely left the cell manipulation room first.

Then, Fang Ziye sent another message to his teacher and started working.

There are two main tasks.

The first is the constant revision and revising of the master's thesis. No matter how meticulous you are in revising your master's thesis, it's never too much; it's something you'll be doing for the rest of your life.

If there are obvious mistakes, they may become a stain on one's record in the future.

The second is preparing for the upcoming doctoral entrance examination, including material preparation and carefully reviewing the examination process.

Although the hospital has already allocated a spot, the procedures still need to be followed, and it's best to go through them smoothly to avoid any arguments between the hospital and other interviewees, which would be undesirable.

After Luo Tingzhu finished her experiment, she stood next to Fang Ziye for a while, looked at the paper Fang Ziye had revised, and did not interrupt. After looking at it for about ten minutes, she sat down at her computer and started typing.

Her school-level research proposal is also nearing completion. She needs to submit it to the school in December to receive funding, and it will require many revisions.

……

After Fang Ziye's experiment ended, he only received eight knowledge points again.

Eight points of knowledge, while not a small amount, didn't give Fang Ziye a satisfying feeling compared to the previous fifty-plus points.

"Tingzhu, could you please proofread my master's thesis and check for typos or basic logical errors?" Fang Ziye extended an invitation.

Such a graduation thesis has little value; it mainly repeats the content of the basic paper he published in the first-tier journal.

This level of depth and experimental procedure is more than sufficient for a master's degree, and even a doctoral degree. However, precisely because of its high value, it requires utmost care and meticulous revision, as it contains a great deal of content.

“Sure, senior.” Luo Tingzhu nodded, and while exiting the lab through the automatic sensor door, she checked her keys, campus card, and cell phone in her chest bag. After tidying them up, she patiently waited for the elevator.

Fang Ziye waited until Luo Tingzhu finished her work before saying, "Okay, thank you. After you've checked, I'll have to ask my senior brothers to help me check it a few more times before I feel at ease."

"I heard that everyone in our cohort is going to be sent for blind review, we can't be careless!"

Since the beginning of the Tianlin era, graduation for all master's and doctoral students has become increasingly difficult. And since the incident involving a senior student two years ago, all master's and doctoral graduates have been required to undergo blind review.

Why? Because a senior student who graduated several years ago had his master's thesis checked after graduation, and it revealed some serious flaws...

It's getting harder and harder!

"Senior brother, you'll definitely be fine."

Luo Tingzhu gripped the straps of her backpack with both hands, her elbows pointing back, the sides of her forearms pressed against her waistline. Even through her wool coat, her slightly slender figure was still visible.

Luo Tingzhu smiled and said, "Senior, your thesis would be perfect for my doctoral graduation. How could it possibly fail the blind review?"

(End of this chapter)