top student at medical school

Chapter 55 Little Crawler (Please add to your favorites and read on!)
Under the microscope, the number of cells that have invaded through the membrane is quite encouraging.

Compared to the normal group, the number of cells treated with Luo Tingzhu was significantly greater. The cells were already stained, and it was clear that the cells treated with Luo Tingzhu were more invasive than the normal group.

This phenomenon indicates that the cells treated by Luo Tingzhu are more aggressive than normal cells.

"Knowledge Points +28.6!"

When the huge amount of knowledge points appeared, Fang Ziye was quite surprised, but at the same time, he felt that it was reasonable.

Although it is currently only an early-stage experiment and has not yet become a systematic research topic, it is a new direction and represents a groundbreaking new knowledge for verification.

"Junior Sister Luo, your preliminary experiments only go as far as the transwell step, right?"

Luo Tingzhu's attention was entirely focused on the view reflected in the mirror, and her tone was slightly tinged with emotion as she replied, "There's no money to be made even if we keep doing this, is there?"

But after she finished speaking, she realized that this tone was not right for Fang Ziye, since she had no money to do experiments and had nothing to do with Fang Ziye.

"Senior Brother Fang, thank you~" Luo Tingzhu turned her head and smiled. Most of her smile was hidden, but when her big, bright eyes and eyelashes fluttered, they were full of kindness, indicating that their owner was very happy at the moment.

"It's alright. I also want to thank you for giving me the opportunity to practice. In any case, I didn't mess it up."

"Then you can do the data analysis first, junior sister. I'll go check on my revived cells." Fang Ziye didn't put any more pressure on Luo Tingzhu. After glancing at her again and seeing that her knowledge points hadn't increased, he decisively moved on to the cell culture incubator.

Each step of the experimental procedure and the cultured cell lines are the original experimental materials. Once they enter the experimental group, the previous cell lines become experimental materials that can no longer be used and need to be disposed of and revived or cultured again.

After completing the scratch test and proving that HK2 does indeed play a role in the metastasis of giant cell tumor of bone, Fang Ziye's next step is to confirm the promoting effect of HK2 on the invasiveness of giant cell tumor of bone.

Luo Tingzhu continued to observe and count the cells in preparation for applying for a research grant.

The preliminary experiments are only part of the experiment. The main purpose is to show that you have already done some experiments and verified the feasibility of your proposed research project. Now all you need is money to get the results.

However, due to limited personal financial resources, I am unable to support the further development of my research project and need support from university-level, city-level, provincial-level, or even national-level research grant groups.

Without money, trying to do research on your own is nothing short of a pipe dream.

Regardless of the type of experiment, the experimental materials, such as cells and the mice and rabbits to be raised, cost more than the monthly expenses of the graduate student. Unless one is extremely wealthy, how could they possibly afford it?

Generally speaking, many universities mention a substantial research start-up fund when recruiting talent. This is to provide you with the funds to prepare for the preliminary research for applying for large-scale research projects.

Research start-up funding is absolutely necessary; otherwise, you won't be able to enter this field.

Make bricks without straw.

Luo Tingzhu was actually able to conduct her current preliminary research by having Professor Deng Yong allocate a small amount of research start-up funds to her. Then, she applied for a small project in the name of Professor Deng Yong or someone else, received funding, conducted some basic experiments, and built up her resume in order to prove her research capabilities and prepare for staying at the hospital.

Compared to Luo Tingzhu, Fang Ziye, a master's student who doesn't have any advantage in job hunting but can stay in the institute to pursue a doctorate, has already secured a general or youth grant from the young supervisor, which becomes the economic pillar that Fang Ziye can build on.

As long as Fang Ziye can produce results, he can freely dispose of the funds in the research project. After reviewing the cells he had cultivated again, the HK2 gene knocked out, Fang Ziye saw tiny, clinging cells growing extremely slowly.

Giant cell tumors of bone don't grow very fast to begin with, so it's estimated that this batch will take about ten days to fully grow before the next round of experiments can begin.

If the experiment fails, then another batch needs to be cultured, which takes another ten days.

Many academic graduate students, whether master's or doctoral, waste most of their time in this way.

After reviewing the cells he had cultured once again, Fang Ziye hesitated for a moment, but decided to upgrade his basic cell experiment skills from level 2 to level 3, just to be on the safe side.

Whether it's the scratch assay or the Transwell invasion assay, these are the simplest components of the cell experiment group. Subsequent experiments such as flow cytometry and other experiments are more complex and require more procedures.

Moreover, Fang Ziye's own experimental time was not entirely sufficient, so he naturally had to be cautious.

After completing the skill point allocation, the large amount of knowledge points I gained today was almost completely used up again, leaving me with only 0.8 knowledge points remaining.
When I returned from the outpatient clinic, the score was 22.1. The cell results increased to 28.6, and the experimental procedures added 0.1 knowledge points, which brought the total to 0.8. Otherwise, it would only be 0.7.
There aren't enough knowledge points, not enough at all. Even with a daily income of 40 to 50 points, it's still not enough.

Fang Ziye saw Luo Tingzhu taking photos repeatedly, recording various data from the preliminary experiments. He estimated that the field of view she was recording was definitely more than just three or five.

"Junior Sister Luo, there's no rush. The application period for school-level projects is usually in December and June, while the application periods for municipal and provincial-level projects are after the general project applications, in April and May," Fang Ziye reassured her.

“Yes, we should try everything. If we can only get a school-level project, we’ll only get a maximum of 20,000 yuan, which will barely cover the cost of this small project. If we can get a city-level project, we might get around 50,000 yuan, which would be great.” Luo Tingzhu said expectantly.

At the same time, he carefully put his petri dish away again, as if to avoid incomplete data recording and missing information. He would have to take more photos after dinner to keep a record.

Next, she will need to perform statistical processing on the data from the invasion experiments.

It's tough for everyone.

If Fang Ziye's mentor had been Deng Yong instead of Yuan Weihong, things might not have been as difficult as Luo Tingzhu's, but they were still not easy.

Having a choice is always a double-edged sword, with both advantages and disadvantages!

Both of them left the cell culture room one after the other, and after disinfecting and sanitation, they straightened their shoes before changing into their own, washing and disinfecting their hands, and waiting for the food delivery to arrive.

After the meal, Fang Ziye didn't have much to do, so he went to the practice room first, while Luo Tingzhu stayed in the laboratory, working hard as a little web crawler to process data for research grant applications.

Fang Ziye glanced at Luo Tingzhu in the elevator, and could only silently pray for her.

The research grants that a teacher applies for are public funds within the teacher's research group. It is almost impossible for someone who has no student relationship with the teacher to get a single penny. This is the difference between a small research group and a large research group.

I need some data support. Thank you all, fellow readers. The book launch is generating a huge return, and we're currently in a critical phase.

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(End of this chapter)