top student at medical school

Chapter 42 Charging Forward Regardless of Everything (Please add to your favorites and read on)

If you were to ask someone in the medical field who is engaged in scientific research, how would you make them angry?

Here are a few good methods.

First, you cheated on him.

Secondly, you turned his cultured cell lines green.

Thirdly, give him a cat instead of the mouse he keeps.

You'll then be able to see his ultimate rampage.

Cell lines, or cell strains, represent the most fundamental and core form of accumulation and inheritance for medical researchers.

Some processed cell lines are not readily available for purchase. The cell lines that are bought are usually unprocessed and not suitable for the research group.

It's like this: if the cell line you buy is silk, then the mature cell line is already cloth, just waiting to be used to weave clothes.

In the cell culture room, Fang Ziye sterilized the experimental supplies with ultraviolet light while warming the PBS buffer and transferred culture medium in a constant temperature water bath at 37 degrees Celsius.

He then explained the basic procedures of cell experiments to his teacher: "Master, the cell scratch experiment is an experimental method to explore the migration ability, repair ability, and interaction of cells."

"It mainly simulates cell migration. Also known as cell movement, cell crawling, or cell motion, it refers to the movement of cells after receiving migration signals or sensing the gradient of certain substances."

"Cell migration is involved in many physiological and pathological activities, such as immunity, inflammation, tumor metastasis, damage repair, and embryonic development."

"The cell scratch assay is a laboratory technique that involves scratching a cell monolayer and periodically capturing images using a time-lapse microscope to study cell migration, repair capabilities, and intercellular interactions..."

"The experimental steps are as follows," Fang Ziye reported one by one, and his report was very rigorous.

After a good ten minutes, Yuan Weihong finally nodded slightly: "Knowing so much, it's not really a disaster."

"Take a look at your lab log." Yuan Weihong took the lab logbook that Fang Ziye had bought online, tapped it, and said, "You need to transcribe everything in this into the lab's lab data logbook from now on."

"Why don't you go and get one?"

"Master, I'm new here and I'm not sure if I can go and get it yet. Making a draft first is fine too." Fang Ziye knew that when conducting experiments, the original data and experimental plans all needed to be saved.

This is crucial evidence that you've conducted experiments and aren't just making things up when publishing papers or completing research projects. Some top journals require this kind of supporting material when accepting articles to prevent academic fraud.

But Fang Ziye wouldn't dare to cause trouble in Professor Deng Yong's laboratory; he hasn't even entered yet.

Experiment logbooks aren't something you can just get whenever you want; Luo Tingzhu hasn't received one yet.

“Then record it first. After you have recorded all the data from the scratch test, write an application and submit it to Professor Deng. He will give it to you.” Yuan Weihong had also gone through the process of becoming a junior master’s or doctoral student, so he knew Fang Ziye’s inner fears.

After checking, Fang Ziye wrote down the time, steps, and plan in a good manner without any mistakes or omissions, so he was relieved.

Unfortunately, the cells that Fang Ziye cultured did not fill the entire culture dish.

Therefore, the cultivation and passage are not yet complete, and cryopreservation and other operations cannot be completed yet.

Of course, Fang Ziye still completed the cell transfer experiment one by one under the personal supervision of Yuan Weihong, and he was very skilled.

Cell transfer, simply put, is the process of moving cells from one culture dish to another to prevent contamination caused by cells remaining in one dish for an extended period. It is one of the fundamental procedures in cell experiments.

It's like kindergarten kids sleeping side-by-side, all close together! Pancreatic enzyme digestion is like waking up all these kids, then randomly dividing them into several classes, and having a certain number of them return to the classroom to sleep again!

Pancreatic enzymes digest the cells, causing them to suspend, which is the process that wakes children up.

"Not bad. You've been preparing for this, haven't you? You've been eyeing this PhD for a long time." Yuan Weihong deliberately drew out his words, his satisfaction mixed with a touch of smugness.

Teachers are naturally happy when their students make progress.

Fang Ziye chuckled, offering neither rebuttal nor explanation.

Having come this far, who wouldn't want to pursue a doctorate? Who wouldn't want to stay in a major hospital?

Yuan Weihong walked out first with his hands behind his back, waiting for Fang Ziye to finish handling the experimental equipment and put the reagents back in the refrigerator and the experimental tools back in the storage cabinet.

With his hands behind his back, he led Fang Ziye, the little chick, out of the lab, saying, "Giant cell tumor of bone is a borderline tumor, although it's not as popular as osteosarcoma."

"But when it comes to publishing articles, it's not about how popular they are, but about doing something in-depth enough. That's how you get high-scoring articles."

"The journal's editorial department mainly looks at the experimental content of your article, not how popular it is. So don't think that just because you're cultivating giant cell tumors, you're inferior to others who have osteosarcomas. That's not how it works."

Fang Ziye nodded: "Master, of course I know. The most important thing is to master the experimental methods and fill the gaps that are currently unknown in the academic world. That's what most research teams are doing now, rather than making breakthroughs."

"The most important thing is to master and learn the methods. With the methods, there are so many unknown gaps in academia now. Just find a gap and fill it in, and that's academic work!"

Today's transfer is complete. Although the number of knowledge points gained wasn't as much as the previous times, it was still 0.5.
Fang Ziye is very much looking forward to the subsequent scratch experiments, hoping they will bring him a faster increase in knowledge points.

"It's good that you understand this. I can't treat you to dinner today. I have to go back to my father-in-law's house with your teacher's wife for dinner. You'll have to take care of yourselves."

"Go back to the practice room early in the evening, and also pay attention to the balance between work and rest."

"Oh, by the way, I heard from people in the lab that Luo Tingzhu has been working really hard lately. Do you know what's going on?" Yuan Weihong suddenly asked, his eyes flashing.

Fang Ziye shook his head: "Junior Sister Luo is very hardworking?"

Fang Ziye hadn't really noticed.

"She usually stays in the clinic until the afternoon, in the practice room until the evening, and then in the lab until the early hours of the morning. She still arrives on time for rounds the next day. This young woman doesn't take care of her health at all."

"I don't know why you're pushing yourself so hard. Just like everyone else, be careful when you see this, don't faint."

"There are very few girls in our orthopedics department to begin with. We only have one in trauma surgery, one in sports medicine, and one in joint surgery. We still need to protect them a little."

“Girls aren’t as resilient as boys,” Yuan Weihong said.

“Okay! I’ll keep an eye on it, Master.” Fang Ziye’s heart tightened.

In this large hospital, who isn't striving hard? Simple yet fulfilling, or perhaps not simple, but still fulfilling.

(End of this chapter)