top student at medical school

Chapter 248 The Great Master's Summoning Technique (Please Subscribe)

The next day.

It's a new workday.

Fang Ziye slept well and arrived at the department around 7:30 after finishing his run early.

Naturally, this time when Fang Ziye came to the department, Director Wu Guonan and others, as well as Shen Tao and Xue Tao, had no other tasks to perform in terms of receiving guests.

So after Fang Ziye arrived, a teasing group was formed.

It was Teacher Shen Tao who started it: "Ziye, people are usually in high spirits when they have good things to do. I think your spirits are a bit too high. You need something to expend your energy."

"Director Xue, what do you think we should do?"

Xue Tao understood Shen Tao's meaning, so she looked at Fang Ziye and said, "Teacher Shen, there's been a strict crackdown lately!"

"Ahem!" Wu Guonan coughed twice after hearing Xue Tao say that.

"There's a limit to jokes. Don't keep bringing up old stories, or people might misunderstand and think we deliberately reported you."

"This matter is quite sensitive to begin with."

"The fact that Dr. Fang was able to win the top prize shows that Dr. Fang was already excellent, not that he became excellent after winning the top prize. On the contrary, the fact that the top prize was awarded to Ziye makes the top prize seem more valuable."

Wu Guonan half-praises and half-states the facts: "After all, others can only see Ziye's appearance, but we need to see the reality clearly."

"If it were a competition of manual repositioning techniques, even if everyone in the entire arena, including all the professors, were to compete, they might not be able to beat Ziye."

This kind of flattery is a bit hard to detect.

If one is not observant enough, they might think that Wu Guonan was giving Xue Tao a warning, telling her to stop bragging.

Fang Ziye's ears were practically calloused; he was somewhat immune to similar remarks.

So I simply listened quietly and didn't say a word.

Since Fang Ziye himself didn't speak, it was difficult to delve deeper into the topic of Fang Ziye.

Soon it was time for shift handover and ward rounds.

When Shen Tao and Xue Tao returned with Fang Ziye and the others after their rounds, Wu Guonan took the initiative to invite them, saying, "Xue Tao, today Director Hong's group has a lot of surgeries scheduled. How about our two groups have a small team-building activity? What do you think?"

At Enshi Central Hospital, there is no specific concept of a "surgery day"; you can schedule an appointment as long as there is surgery available.

Both Wu Guonan and Xue Tao had the task of welcoming and seeing off guests.

Director Hongdu has retired, and so have all his contemporaries. With nothing much to do, Director Hongdu spends his time arranging surgeries in the department.

“I’m fine with anything. We only have one minor surgery today, a schwannoma. I plan to have Dr. Fang take Mao Mao to have it done.”

"Teacher Shen, is it convenient for you?" Xue Tao asked Shen Tao, not daring to make a decision for Shen Tao.

"I'm fine with anything," Shen Tao nodded, secretly pleased.

It should be said that the atmosphere in Enshi is much better when Yuan Weihong is not around; even the air feels fresher.

"Well then," Xue Tao said, seeing that Shen Tao had agreed. She was about to reply.

"Brother Tao, I need to go to the lab later. I can come over around noon, but I might be a little late." Fang Ziye didn't refuse, but explained that he did have a task to do.

Wu Guonan and others were aware of Fang Ziye's intention to conduct animal experiments, and naturally understood.

"What kind of animal experiments are you doing? I'm free anyway, how about we go have some fun together?" Shen Tao was a little curious about what Fang Ziye was up to these days.

However, Fang Ziye could not satisfy Shen Tao's curiosity.

"Teacher Shen, I can go by myself, it's not good to trouble you." Fang Ziye didn't explain further.

Shen Tao can't get into this research group right now because the Provincial People's Hospital doesn't have a registered laboratory at the sanatorium, so he can't get in.

Fang Ziye was able to get in because Professor Zu had specifically requested a spot for him, and currently, a very small team from Zhongnan Hospital has also entered the laboratory.

This gave Fang Ziye the opportunity to sneak in.

Fang Ziye doesn't plan to let Shen Tao know about these things right now. As for when Shen Tao will find out, that's something for teacher Yuan Weihong to consider.

A minor surgery for a schwannoma is not particularly difficult; it is essentially an organic combination of incision and nerve exploration.
Fang Ziye was the surgeon, but Xue Tao didn't dare to be careless. She followed him through the entire surgery and only left after Fang Ziye finished.

Fang Ziye completed these operations at just 9:20.

After the surgery, Xue Tao, fearing that Fang Ziye might misunderstand, added, "Ziye, it's not that I'm worried about you taking Mao Mao through surgery alone."

“I’ve been feeling a bit uneasy lately, and the atmosphere in Enshi is a bit strange, so we shouldn’t let our guard down.”

"Try to do a good job of each procedure."

Fang Ziye washed his hands and said with a smile, "Brother Tao, I feel more at ease knowing you can come."

"I'm afraid you'll think I'm too bad, and that watching my gameplay is too annoying."

"You're just being modest," Xue Tao said, pointing at Fang Ziye's face and playfully flicking a little water on it.

Xue Tao wasn't exactly a goofball, but she did have a playful side occasionally, vividly embodying the saying "a man is a boy until death." Fang Ziye didn't linger. After changing his clothes and saying goodbye to Xue Tao, he took a taxi directly to the animal laboratory after leaving the hospital…

This animal laboratory is an absolute SPF-rated facility.

As soon as Fang Ziye arrived, the staff member in charge of animal care said, "Oh, you've finally arrived. If you hadn't come, I would have called you."

“Most of your mice have miscarried.” The staff member, who was probably in his forties, was very concerned at this moment.

He knows a little bit, but not much. He is not a very professional researcher. Even professionals would not know Fang Ziye's current research direction.

All he knew was that Fang Ziye wanted him to raise a batch of pregnant rats, preferably ones that could reproduce.

"Hmm! It's alright, Uncle, it was all expected." Fang Ziye wasn't surprised.

What I was trying to do was knock out the HK2 gene in embryonic stem cells. The more miscarriages occurred, the more precise my operation was, resulting in the embryonic stem cells being unable to utilize energy after being processed and transplanted into the mouse's uterus.

Or it could be due to unstable embryonic cells, leading to miscarriage and necrosis of embryonic cells.

If both pregnancies are successful and mice are produced.

There's no need to look. Fang Ziye probably didn't even scratch the surface of the other party. The other party's cells are still very healthy and normal SPF-grade embryonic mouse cells, without any treatment.

"Now only two of them are still pregnant."

"It's like you've wasted all your money." The uncle knew the price was high, so he loudly reminded Fang Ziye.

"Do you want to continue?" Although the laboratory encouraged them to let the experimental technicians bring in more business, Fang Ziye's experiment had 11 mice, and nine of them had died.

According to Fang Ziye's current plan to establish a few native models of pregnant mice, the total cost for each mouse is around 30,000 yuan, which is equivalent to 330,000 yuan.

All 260,000 to 270,000 yuan went down the drain.

"Two more are pregnant? Really?" Fang Ziye immediately stopped, turned around, and asked urgently.

Such a high miscarriage rate proves that there was nothing wrong with my operation.

If the operation goes smoothly, then the most likely outcome is that I might actually have a chance to be among the first batch of people to create a model by chance.

In fact, the biological significance of establishing an animal model with HK2 knockout ends the moment it is born and its HK2 knockout genotype is confirmed.

In the biochemical explanation of carbohydrate metabolism, HK2 hexokinase is the first link in carbohydrate metabolism and almost controls the source of all energy metabolism. This is also the link with the most in-depth understanding of energy metabolism.

Logically speaking, if HK2 is missing, then life will inevitably end.

Aerobic respiration and glycolysis are completely out of the question.

However, if life can survive after HK2 is knocked out, then there is only one reason: our current understanding of energy generation is relatively limited.

There may be other pathways that allow sugar to undergo glycolysis, or other pathways that allow cells to utilize the energy we absorb.

"Of course it's true." The uncle's eyes widened when he heard Fang Ziye's doubt.

"I've raised mice for so many years, I can tell whether they're pregnant or not just by touching them," the uncle said with a hint of reproach.

Fang Ziye didn't distrust the older man's ability to determine if someone was pregnant.
Although this is the early stage of a mouse's development, it is not possible to observe it visually.

Instead?

Fang Ziye rushed over and, as the uncle explained, saw that the two rats might still be alive inside their bellies.

But at this point, Fang Ziye couldn't do anything.

The creation of a gene-knockout organism actually takes place during the embryonic stem cell stage.

After this stage, most of the remaining time is spent waiting. Waiting for it to either miscarry, die, or be born into this world.

Even so, Fang Ziye was still very excited.

Fang Ziye immediately ran out of the animal room, washed and disinfected his hands, and after doing all that, he quickly took his phone from the locker and started calling his teacher.

The rules in this laboratory are very strict; cell phones are not allowed inside.

The control panel here has high-density recording equipment, so that the videos, images, or measurement data you operate can all be recorded.

You just operate it and leave the rest to the equipment. It's much more professional than the photos taken with your phone, and it also has many miniature cameras that can capture special close-up shots.

“Master, Master, there are pregnant ones too. There are two pregnant mice.” Fang Ziye recounted the phenomenon and outcome that made him happy.

"Huh?" Yuan Weihong took a break from his surgery to answer the phone!
Now that all the undergraduate courses have ended and the exams are over, he finally has time to attend the surgery day. Otherwise, he would usually be in the classroom right now.

“There are two pregnant mice. Master.” Fang Ziye took two calm breaths.

"No, Ziye! Even if species isn't the problem, how about we find a species of suitable size first?"

"What does it have to do with you that this mouse is pregnant?"

“Master, animal models, the establishment of animal experimental models after HK2 gene knockout, a question I was asked during my defense,” Fang Ziye explained.

On Yuan Weihong's end, his phone had clearly fallen, making a crisp cracking sound.

(End of this chapter)