top student at medical school

Chapter 117 Um? I have to go now (Please subscribe)
Fang Ziye started eating first, and finished his lunch first.

After finishing his meal, he stood up, threw the lunchbox into the trash can at the bottom of the stairs, and unlocked the lab door with his fingerprint.

At this moment, Liu, the senior student whom Luo Tingzhu had invited from some department's lab, was scrolling through his phone with an air of nonchalant confidence. When he saw someone open the door, he looked up, smiled at Fang Ziye, and that was his way of greeting him.

Fang Ziye didn't recognize him, at least not as a senior in orthopedics, so he didn't go up to say anything.

It's perfectly reasonable for Luo Tingzhu to ask someone for advice.

This senior student's initiative was brave and direct, and there's nothing wrong with that.

As long as he doesn't have any bad intentions or come up with any crooked or evil ideas, Fang Ziye has no grounds to interfere and should just focus on doing his own thing.

Naturally, Luo Tingzhu hoped that Fang Ziye could show off a little. From her perspective, she hoped that this Senior Brother Liu would not bother her in the future. Fang Ziye could not agree to this, nor could he take the initiative to provoke her. He should just do his job well.

Otherwise, if you encounter a tough opponent, you might end up making a fool of yourself and becoming a laughingstock.
Quietly do your job well and demonstrate certain abilities.

If you want to come to the orthopedic lab to help with experiments, the lab welcomes you, but you can't expect to show off.

Even if he were a formidable fighter, if Yuan Weihong or Qin Geluo were to come, he would have no choice but to slink back home.

Of course, Professor Deng Yong's practical skills in basic experiments may not be very good, but there is still Associate Professor Hong Zili!
Associate Professor Hong Zili's research background cannot be directly compared with that of Professor Liu Huanglong in hand surgery. However, in the field of his specialty, Associate Professor Hong Zili is currently an academic giant, even more outstanding than Liu Huanglong. It's just that his professional ability is not as strong. He is an absolute top-notch researcher in the field of surgery at Zhongnan Hospital.

Even if a full professor like Professor Deng Yong can be without students, Associate Professor Hong Zili cannot be without master's and doctoral students. These are Professor Deng Yong's own words.

Fang Ziye needs a cell manipulation table, and so does Luo Tingzhu.

Moreover, Luo Tingzhu's cell manipulation station was booked by Fang Ziye, and the time was only a dozen minutes later than Fang Ziye's.

There are three operating tables in the cell manipulation room.

Fang Ziye went in fully equipped, took out the culture medium and PBS buffer that he had preheated even earlier than Luo Tingzhu, and then disinfected all the reagents, both heatable and non-heatable, with alcohol before putting them into the operating table.

When Luo Tingzhu brought Senior Brother Liu into the laboratory, Fang Ziye had already started operating the equipment.

Fang Ziye lit the alcohol lamp in the operating table, turned on the exhaust fan, and lowered the glass of the cell operating table before starting to digest the cells in the culture flask with trypsin.

Pancreatic enzyme digestion is a necessary step in many procedures.

When cells are in a culture flask, they adhere to the wall. They need to be digested and then suspended so that they can be separated from the reagents by centrifugation. Then they can be passaged, dispensed, and transferred to culture dishes and culture plates. This is essentially a procedural operation.

This action can be compared to stepping out of your home. Whether you're going downstairs to eat, buy fruit, or do something else, you first have to step out the door.

This step is very simple and doesn't involve much operation; the centrifuge is the main contributor.

Fang Ziye removed the pancreatic enzyme suspension to avoid over-digestion that could kill the cells.

Because cells that adhere to the wall are like elementary school students sleeping soundly in a dormitory, pancreatic enzymes are like throwing a smoke bomb into the dormitory.

But the smoke bombs only got them out of the dorm room, not trapped them inside and suffocated them.

However, after Fang Ziye completely suspended the separated cell populations and re-proportioned them,

Fang Ziye quickly counted the suspended cells.

The cell counting procedure will not be performed in the cell culture room for the time being. We need to mix some reagents and run a flow cytometer to determine the concentration.

Cell concentrations within the same batch of samples can be equivalent.

Just like when you drink a beverage, the first sip and the last sip should theoretically have the same sweetness or taste.

After counting the samples, Fang Ziye did not take them back. Instead, he sealed them and threw them directly into a biological waste bag.

But once the concentration was known, Fang Ziye began his operation.

The principle of diluting a sample is actually very simple: if you add 1 ml of sample to 99 ml of water, it is diluted by 100 times. If you add 1 ml of sample to 999 ml of liquid, it is diluted by 1,000 times.

When conducting concentration gradient experiments, this process needs to be repeated once, usually by a 10-fold dilution each time.

Normally, you only need to prepare the liquid volume in advance: 9ml, 99ml, 999ml.

Then add 1ml of cell suspension, mix well, and then take 1ml from the 9ml+1ml mixture and transfer it to 99ml.

It's just a matter of replacing a few pipette tips at most.

But Fang Ziye today doesn't operate that way.

He adjusted the pipette to 200µL and then squeezed out 1µL, 10µL, and 100µL directly into the culture dish.

The scale was not adjusted, and the pipette was not replaced.

This tactic was definitely Yuan Jianda's doing.

After all, this is essentially blind operation. If you're using a 200ul pipette, how can you possibly squeeze out 1ul of liquid without a fixed quantity? That's ridiculous.

But that's exactly what Fang Ziye did.

Naturally, neither Senior Brother Liu nor Luo Tingzhu, who were operating the device nearby, noticed these minor details. All they saw was that Fang Ziye hadn't changed the pipette tip and was just dabbing and dabbing like a chicken pecking at rice.

After a while, Fang Ziye added 999, 990, and 900 μL of pure culture medium liquid to each of the different samples.

Then a few more test tubes were added, and the cell concentration was diluted again using the most standardized procedures.

This time, Fang Ziye aimed to find the optimal cell concentration level for operation and ultimately select the most suitable concentration for the experiment.

After all, no one else has done this research on the relationship between miRNA and HK2 in giant cell tumor of bone that Fang Ziye is currently working on. Fang Ziye referenced the optimal concentration of HK2 in other tumor cells, but failed to obtain the best images, so he had to find it himself.

Everything is unknown, even the parameters are unknown, which is the difficulty of some basic experiments.

It doesn't cost much, it just takes time...

After performing this operation several times, Fang Ziye first covered a 24-well cell culture plate and then placed it into a constant temperature cell culture incubator.

Fang Ziye's next step is to address this concentration gradient difference in another giant cell tumor cell line of bone.

This is because the optimal response concentration may differ between different cell lines. It's similar to how, in clinical practice, even with the same giant cell tumor of bone, different subtypes require different neoadjuvant chemotherapy regimens before and after surgery.

It was all learned through trial and error.

Fang Ziye's experiments were aimed at publishing in high-quality journals with an impact factor of at least 15, not just ordinary journals. Naturally, he had to make the most comprehensive and thorough preparations.

Furthermore, after finding the order of magnitude, Fang Ziye still needs to find the preconditions for the optimal response, which is 1-9, to see if there are any differences at the same order of magnitude.

Once the optimal parameters of 1-9 are reached, and there is an order of magnitude of optimal solution, there is no need to search further.

The giant cell tumor cell lines used by Fang Ziye include GCT-0404, GCT1, and GCTSC. (Note: These cell lines are entirely original; knowledgeable readers should not attempt to correlate them with real-world applications.)
After Fang Ziye left, Liu, the senior brother who had been watching, looked at Luo Tingzhu thoughtfully and asked curiously, "Junior Sister Luo, is this your senior brother or your junior brother? Are they always so careless when doing experiments?"

"I think he was planning to perform a standard curve operation just now, just blindly operating it?"

To those who don't understand, Fang Ziye's actions seem like nothing more than adding a few things, but to professional researchers conducting cell experiments, what are all these things?
If your teacher sees this, he'll skin you alive.

Why do you insist on making things difficult for yourself when there's a standard procedure available? However, based on experience, 1 ml of water is generally 20 to 25 drops, so one drop of water is 0.04 to 0.05 ml. If you use a regular dropper, it's about 0.05 ml, and if you use a burette, it's about 0.04 ml.

Old Liu himself hadn't calculated how many ul (µl) it would be to dispense a drop using a 100µl pipette tip, but anyway, if his student dared to do that, he'd either slap them or yell at them until they were depressed for two or three days and kicked out of the lab to reflect on their actions.

"Oh, Senior Brother Fang? I don't know him." Luo Tingzhu chuckled inwardly, but kept a serious expression.

"Brother Fang's operating skills are much better than mine. When I was learning from Brother Fang before, he always operated step by step, but recently I don't know much about it."

Senior Brother Liu didn't say anything more. After completing the cell dispensing process, he took Luo Tingzhu to construct the miRNA silencing cell line.

This will require a certain amount of waiting time.

Slightly boring.

Perhaps Luo Tingzhu had been nagging Liu Shixiong for a while, so Liu Shixiong was a little curious about what exactly this Fang Ziye was, since he hadn't understood it at all just now.

So, after he and Luo Tingzhu came out, he asked, "Junior sister, could you show me around? I've never been to your orthopedics lab before."

Seeing that the laboratory wasn't particularly large, even slightly smaller than the laboratory in his urology department, Senior Brother Liu made this suggestion.

Luo Tingzhu did not refuse. Guests are guests, and no matter how shameless they are or how they came, as long as they do not force their way in, they should be treated with proper hospitality.

Senior Brother Liu is considered an outsider to the lab; she brought him in, so she can't let him leave freely. She has to follow him.

Instead of following Fang Ziye to see him, Senior Brother Liu wandered around, hoping to run into someone he knew. To his surprise, he did indeed run into someone he knew, who was his classmate and currently a doctoral student in thoracic surgery.

His name is Qian Qiaofeng, and his nickname is Gang Leader.

Qian Qiaofeng was tall and thin with delicate features. He also wore glasses. He ran into Senior Brother Liu and Luo Tingzhu as he was leaving.

Seeing Luo Tingzhu following beside Senior Brother Liu with his hands behind his back, as if he understood something, he asked, "Brother Yuan, are you here for an inspection? Or to pay a visit?"

Liu Tengyuan is the name of Senior Brother Liu.

"Boss, you? You're in this lab?" Liu Tengyuan's heart tightened the moment he saw Qian Qiaofeng.

Qian Qiaofeng is a doctoral student in thoracic surgery. He is a specialized graduate student, but he is also very good at conducting experiments. Among the doctoral students in his cohort, Qian Qiaofeng is a perennial recipient of the National Scholarship.

To win the national scholarship, it's not just a professional evaluation; it involves evaluating all medical students, including internal medicine, surgery, obstetrics and gynecology, pediatrics, dermatology, and neurology. Only those in the top 10% have a chance to receive the national scholarship, which basically requires a significant output each year.

"Yes, my master conducts experiments here!"

"Tingzhu, should you introduce them, or should I have Brother Yuan introduce them?" Qian Qiaofeng put down the tray containing the experimental equipment and reagents he was holding and started chatting.

Luo Tingzhu quickly explained, "Senior Brother Qian, this is Senior Brother Liu. He came to help me with the experiment. He wants to visit our lab."

Qian Qiaofeng's gaze softened slightly, and he looked at Liu Tengyuan with a thoughtful expression.

When Liu Tengyuan saw Qian Qiaofeng, his gaze softened slightly. What the hell? How did he just barge into the lab of Hong Zili, a leading figure in thoracic surgery research? Wasn't this like walking headfirst into a brick wall?

Liu Tengyuan quickly explained, "Chief, that's not fair of you. Your junior sister is in trouble and you don't even help her. You have to call me from downstairs all the way."

"Can't you read less comics and anime?"

Qian Qiaofeng, standing sideways with the tray on his head, said, "What the hell am I supposed to watch? I haven't watched any anime for two weeks, let alone watch comics."

"So busy I'm dying..."

After this back and forth, Luo Tingzhu was finally free. Qian Qiaofeng and Liu Tengyuan were classmates, and since Liu Tengyuan wanted to see it, Qian Qiaofeng couldn't refuse, so he personally led Liu Tengyuan around.

Liu Tengyuan inwardly groaned, secretly resenting Qian Qiaofeng for being so unkind or heartless. "Do I need you to show me around?" he thought.

However, after a while, when Qian Qiaofeng slowly introduced some of his senior and junior brothers to Liu Tengyuan, Liu Tengyuan's expression gradually became serious and calm.

"Wow, the equipment in this lab is amazing! It seems even more comprehensive than a typical provincial key laboratory. There are so many cell storage refrigerators!" Liu Tengyuan exclaimed in amazement at the entrance of the cell cryopreservation room.

"It's alright, it's enough for temporary storage, but sometimes it's still not enough. There are quite a few people in the lab, and too much stuff."

"Look, this cabinet belongs to my senior brother. He takes up half of it, with more than ten different cell lines..."

"and this……"

Liu Tengyuan nodded: "Professor Hong is really impressive. Can you be promoted to professor this year?"

"It's probably difficult. My teacher has devoted most of his time to scientific research and didn't make it to the chief physician stage. If he can be promoted to chief physician this year, he can be promoted to professor," Qian Qiaofeng explained.

Then, the two walked to an operating room, and Qian Qiaofeng immediately paused: "This is Professor Deng's student in orthopedic trauma surgery, our junior brother, he's absolutely amazing!"

"His basic experimental skills are even a little bit more advanced than my senior's."

"Look, what do you think is the success rate of all the operations he's currently performing?"

"An 80% success rate?" Liu Tengyuan said, which was a rather exaggerated figure.

Many people may not get results on the first try and need to repeat the experiment two or even three times, constantly making adjustments.

"Currently, it remains at 98%. We have performed at least 50 small operations, and so far, we have only repeated them once. This 98% figure was calculated more than ten days ago."

"That's awesome. My senior said that after he graduates, if I ever have trouble with any experiments, I can ask him for help."

"Look, that guy is also in orthopedics, Dong Wenqiang, he's in the same cohort as me, an academic PhD, you probably haven't met him much."

"He's a rather reclusive person. He could have won the national scholarship, but he was too lazy to participate. His family doesn't lack that kind of money, and he just wants to work honestly and diligently. He has published more articles than me. He published two articles with an impact factor of over 6 during his doctoral studies, and I estimate that he has one more to submit to other journals."

“This is…” Qian Qiaofeng knew it like the back of his hand.

After walking around for a while, Liu Tengyuan suppressed his embarrassment and quickly returned to Luo Tingzhu's side.

It wasn't good to leave right away; I'd already promised to help Luo Tingzhu finish the experiment, so I had no choice but to bite the bullet and do it.

However, after he finished processing the cell line, Liu Tengyuan quickly said, "Well, junior sister, the operation here is almost done. I have something to do, so I'll be leaving now."

"Thank you, senior brother. If you need help with experiments next time, please call me." Luo Tingzhu quickly got up and followed, speaking politely.

In the past, she might have offered to treat her fellow students to a meal, but now she wouldn't. The meals weren't good, she couldn't afford to pay for them, and she'd only incur favors – it wasn't worth it.

Liu Tengyuan quickly grabbed his Armani backpack and left, not daring to linger any longer.

However, Luo Tingzhu lingered on the words "Liu Tengyuan's backpack" a few times, then pursed her lips, turned around, and murmured to herself as she did so.

"Dad said that when dating, you shouldn't look for someone from a family that's too well-off. It's better to look for someone who's about the same. Ideally, you should look for someone whose family has ten to twenty times the amount of pocket money in their bank account. If it's too high, it's hard to be sincere; if it's too low, you'll have a hard time making a living."

"For the average person, the bag they're willing to buy without batting an eye is probably between one-thousandth and one-ten-thousandth of their entire savings. Young people might be even more extravagant..."

"But this card has 850,000 on it, which doesn't seem like it'll be easy to find."

"Never mind, never mind..." Luo Tingzhu walked quickly into the cell culture room to take photos as soon as possible.

(End of this chapter)